A Graphene Oxide˙Streptavidin Complex for Biorecognition – Towards Affinity Purification (pages 2857–2865)
Zunfeng Liu, Linhua Jiang, Federica Galli, Igor Nederlof, René C. L. Olsthoorn, Gerda E. M. Lamers, Tjerk. H. Oosterkamp and Jan Pieter Abrahams
Article first published online: 13 JUL 2010 | DOI: 10.1002/adfm.201000761
A novel method is described for preparing graphene oxide (GO)•streptavidin complexes that can be used for affinity purification. The complex shows strong biotin-recognition capability and excellent loading capacity. Capturing biotinylated DNA, fluorophores, and Au NPs on the GO•streptavidin complexes demonstrates its usefulness as a docking matrix for affinity purification, specifically well suited for direct imaging by EM and AFM.