Conditional DNA-Protein Interactions Confer Stimulus-Sensing Properties to Biohybrid Materials (pages 2861–2867)
Erik H. Christen, Maria Karlsson, Michael M. Kämpf, Ronald Schoenmakers, Raphael J. Gübeli, Hanna M. Wischhusen, Christian Friedrich, Martin Fussenegger and Wilfried Weber
Article first published online: 25 MAY 2011 | DOI: 10.1002/adfm.201100731
A biohybrid hydrogel is synthesized for the drug-inducible release of biopharmaceuticals. The hydrogel consists of linear polyacrylamide crosslinked by the interaction of the tetracycline repressor scTetR with its target DNA sequence tetO. Addition of tetracycline dissociates the protein-DNA interaction and triggers the release of a previously embedded payload protein.