Enhanced Enzymatic Thermal Stability and Activity in Functionalized Mesoporous Silica Monitored by 31P NMR



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Organophosphorus hydrolase (OPH) is immobilized on ammonium-modified mesoporous silica particles. Thermal stability and activity are measured with a 31P NMR assay of the conversion of paraoxon (toxic) to its non-toxic hydrolysisproduct. After immobilization, OPH is significantly more active at room temperature and retained activity even after being heated to 45 °C for 1 month.