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Efficient Cell Reprogramming Using Bioengineered Surfaces

Authors

  • David Horna,

    1. Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià-Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain
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  • Juan Carlos Ramírez,

    1. Department of Cardiovascular Development and Repair, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain
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  • Anna Cifuentes,

    1. Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià-Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain
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  • Antonio Bernad,

    1. Department of Cardiovascular Development and Repair, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain
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  • Salvador Borrós,

    1. Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià-Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain
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  • Manuel A. González

    Corresponding author
    1. Department of Cardiovascular Development and Repair, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain
    • Department of Cardiovascular Development and Repair, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain.
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Abstract

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A novel method for cell reprogramming is been developed by immobilizing nucleic acid transfer vectors containing free amino groups, like lentiviral particles, onto pentafluorophenyl methacrylate (PFM)-modified surfaces obtained by PFM grafting affter Ar plasma treatment. This technique is able to reprogram murine somatic cells into pluripotent cells at high efficiencies. We call these modified surfaces cell reprogramming surfaces, or CRS.

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