Delivery of Ricin Toxin A-Chain by Peptide-Targeted Mesoporous Silica Nanoparticle-Supported Lipid Bilayers

Authors

  • Katharine Epler,

    1. Center for Micro-Engineered Materials, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA
    2. Ceramic Processing and Inorganic Materials Department, Sandia National Laboratories, P.O. Box 5800, MS 1349, Albuquerque, NM 87185, USA
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  • David Padilla,

    1. Center for Micro-Engineered Materials, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA
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  • Genevieve Phillips,

    1. University of New Mexico Cancer Center, 1 University of New Mexico, MSC 07-4025, Albuquerque, NM 87131, USA
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  • Peter Crowder,

    1. Department of Chemical Engineering, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA
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  • Robert Castillo,

    1. Center for Micro-Engineered Materials, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA
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  • Dan Wilkinson,

    1. Center for Micro-Engineered Materials, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA
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  • Brian Wilkinson,

    1. Center for Micro-Engineered Materials, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA
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  • Cameron Burgard,

    1. Center for Micro-Engineered Materials, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA
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  • Robin Kalinich,

    1. Ceramic Processing and Inorganic Materials Department, Sandia National Laboratories, P.O. Box 5800, MS 1349, Albuquerque, NM 87185, USA
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  • Jason Townson,

    1. Center for Micro-Engineered Materials, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA
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  • Bryce Chackerian,

    1. University of New Mexico Cancer Center, 1 University of New Mexico, MSC 07-4025, Albuquerque, NM 87131, USA
    2. Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, 1 University of New Mexico, MSC 08-4660, Albuquerque, NM 87131, USA
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  • Cheryl Willman,

    1. University of New Mexico Cancer Center, 1 University of New Mexico, MSC 07-4025, Albuquerque, NM 87131, USA
    2. Department of Pathology, University of New Mexico Health Sciences Center, 1 University of New Mexico, MSC 08-4640, Albuquerque, NM 87131, USA
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  • David Peabody,

    1. University of New Mexico Cancer Center, 1 University of New Mexico, MSC 07-4025, Albuquerque, NM 87131, USA
    2. Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, 1 University of New Mexico, MSC 08-4660, Albuquerque, NM 87131, USA
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  • Walker Wharton,

    1. University of New Mexico Cancer Center, 1 University of New Mexico, MSC 07-4025, Albuquerque, NM 87131, USA
    2. Department of Pathology, University of New Mexico Health Sciences Center, 1 University of New Mexico, MSC 08-4640, Albuquerque, NM 87131, USA
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  • C. Jeffrey Brinker,

    1. Center for Micro-Engineered Materials, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA
    2. Department of Chemical Engineering, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA
    3. Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, 1 University of New Mexico, MSC 08-4660, Albuquerque, NM 87131, USA
    4. Self-Assembled Materials Department, Sandia National Laboratories, P.O. Box 5800, MS 1349, Albuquerque, NM 87185, USA
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  • Carlee Ashley,

    Corresponding author
    1. Biotechnology and Bioengineering Department, Sandia National Laboratories, P.O. Box 969, MS 9292, Livermore, CA 94551, USA, Fax: (925) 294-3020
    2. University of New Mexico Cancer Center, 1 University of New Mexico, MSC 07-4025, Albuquerque, NM 87131, USA
    • Biotechnology and Bioengineering Department, Sandia National Laboratories, P.O. Box 969, MS 9292, Livermore, CA 94551, USA, Fax: (925) 294-3020
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  • Eric Carnes

    Corresponding author
    1. Department of Chemical Engineering, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA, Fax: (505) 272-7336
    2. University of New Mexico Cancer Center, 1 University of New Mexico, MSC 07-4025, Albuquerque, NM 87131, USA
    • Department of Chemical Engineering, University of New Mexico, 1 University of New Mexico, MSC 01-1120, Albuquerque, NM 87131, USA, Fax: (505) 272-7336.
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Abstract

original image

Mesoporous silica nanoparticle-supported lipid bilayers, or “protocells”, exhibit a high loading capacity, enhanced colloidal stability, and peptide-directed, cell-specific uptake, making them especially well-suited for targeted delivery of protein toxins to cancer. Protocells loaded with ricin toxin A-chain (RTA) and targeted to hepatocellular carcinoma cause complete cell death at 30 pM of RTA without affecting the viability of control hepatocytes.

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