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Microfluidic Cell Sorter (μFCS) for On-chip Capture and Analysis of Single Cells

Authors

  • Jaehoon Chung,

    1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Huilin Shao,

    1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA
    2. Harvard Biophysics Program, Harvard Medical School, Boston, MA 02115, USA
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Thomas Reiner,

    1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA
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  • David Issadore,

    1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA
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  • Ralph Weissleder,

    Corresponding author
    1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA
    2. Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    • Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA.
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  • Hakho Lee

    Corresponding author
    1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA
    • Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA.
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Abstract

original image

A low-cost and rapid microfluidic cell sorter (μFCS) for circulating tumor cell (CTC) detection, culture and analyses is developed. Based on size separation and molecular characterization, μFCS efficiently enriches CTCs from unprocessed whole blood, allows on-chip culture and molecular profiling, and provides cell retrieval for subsequent analyses. The potential clinical application of the technology is demonstrated by capturing and genetically analyzing CTCs in tumor-bearing mice.

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