Deposition of Triamcinolone Acetonide and Its Effect on Soft Tissue Topography

Authors

  • Celimar Valentin-Rodriguez,

    1. Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Drive, West Lafayette, IN, 47907, USA
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  • Tongalp H. Tezel,

    1. Kentucky Lions Eye Institute, Department of Ophthalmology and Visual Sciences, University of Louisville School of Medicine, 301 East Muhammad Ali Boulevard, Louisville, KY, 40202, USA
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  • Albena Ivanisevic

    Corresponding author
    1. Department of Materials Science and Engineering, North Carolina State University, 911 Partner's Way, Raleigh, NC, 27695, USA, UNC/NCSU Joint Department of Biomedical Engineering, North Carolina State University, 911 Oval Drive, Raleigh, NC, 27695, USA
    • Department of Materials Science and Engineering, North Carolina State University, 911 Partner's Way, Raleigh, NC, 27695, USA, UNC/NCSU Joint Department of Biomedical Engineering, North Carolina State University, 911 Oval Drive, Raleigh, NC, 27695, USA.
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Abstract

Bimodal imaging is utilized to characterize the topography of human tissue samples. The deposition of triamcinolone acetonide (TA) on various surfaces – including surgical human inner limiting membrane (ILM) samples and collagen fibrillar sheets – was studied. Changes in composition were well defined with bimodal imaging when TA deposition was examined on mica. TA sedimentation resulted in observable changes in ILM topography when compared to collagen fibrillar sheets. The heterogeneous chemical and topographical features of the ILM tissues promoted the TA crystallization compared to the flatter and homogeneous collagen surfaces. Higher spatial resolution was achieved by imaging ILM samples in the new bimodal imaging mode. The most apparent difference was observed in the imaging of ILM samples which had been exposed to the steroid TA. The study demonstrated the usefulness of bimodal imaging to evaluate tissue samples.

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