Regulating Specific Growth Factor Signaling Using Immobilized Branched Ligands

Authors

  • Michael W. Toepke,

    1. Departments of Biomedical Engineering and Orthopedics & Rehabilitation, University of Wisconsin, 5009 Wisconsin Institutes of Medical Research, 1111 Highland Ave., Madison, WI 53705, USA
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Nicholas A. Impellitteri,

    1. Departments of Biomedical Engineering and Orthopedics & Rehabilitation, University of Wisconsin, 5009 Wisconsin Institutes of Medical Research, 1111 Highland Ave., Madison, WI 53705, USA
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Sheeny K. Lan Levengood,

    1. Departments of Biomedical Engineering and Orthopedics & Rehabilitation, University of Wisconsin, 5009 Wisconsin Institutes of Medical Research, 1111 Highland Ave., Madison, WI 53705, USA
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  • Derek S. Boeldt,

    1. Obstetrics & Gynecology, University of Wisconsin
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  • Ian M. Bird,

    1. Obstetrics & Gynecology, University of Wisconsin
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  • William L. Murphy

    Corresponding author
    1. Departments of Biomedical Engineering and Orthopedics & Rehabilitation, University of Wisconsin, 5009 Wisconsin Institutes of Medical Research, 1111 Highland Ave., Madison, WI 53705, USA
    • Departments of Biomedical Engineering and Orthopedics & Rehabilitation, University of Wisconsin, 5009 Wisconsin Institutes of Medical Research, 1111 Highland Ave., Madison, WI 53705, USA.
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Abstract

original image

VEGF-binding peptide ligands are incorporated into hydrogel microspheres and reduce the amount of growth factor in solution. VEGF binding affinity is enhanced by creating ligands with a dimer structure. The spheres are able to knock down VEGF-mediated HUVEC growth and reduce calcium signaling. The binding interaction is reversible, allowing the spheres to be used as a VEGF delivery vehicle.

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