Well-Defined Degradable Cationic Polylactide as Nanocarrier for the Delivery of siRNA to Silence Angiogenesis in Prostate Cancer

Authors

  • Chih-Kuang Chen,

    1. Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260-4200, USA
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Wing-Cheung Law,

    1. Institute for Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY 14260-4200, USA
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Ravikumar Aalinkeel,

    1. Department of Medicine, Division of Allergy, Immunology, and Rheumatology, University at Buffalo, State University of New York, Buffalo General Hospital, Buffalo, NY 14203, USA
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  • Bindukumar Nair,

    1. Department of Medicine, Division of Allergy, Immunology, and Rheumatology, University at Buffalo, State University of New York, Buffalo General Hospital, Buffalo, NY 14203, USA
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  • Atcha Kopwitthaya,

    1. Institute for Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY 14260-4200, USA
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  • Supriya D. Mahajan,

    1. Department of Medicine, Division of Allergy, Immunology, and Rheumatology, University at Buffalo, State University of New York, Buffalo General Hospital, Buffalo, NY 14203, USA
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  • Jessica L. Reynolds,

    1. Department of Medicine, Division of Allergy, Immunology, and Rheumatology, University at Buffalo, State University of New York, Buffalo General Hospital, Buffalo, NY 14203, USA
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  • Jiong Zou,

    1. Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260-4200, USA
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  • Stanley A. Schwartz,

    Corresponding author
    1. Department of Medicine, Division of Allergy, Immunology, and Rheumatology, University at Buffalo, State University of New York, Buffalo General Hospital, Buffalo, NY 14203, USA
    • Department of Medicine, Division of Allergy, Immunology, and Rheumatology, University at Buffalo, State University of New York, Buffalo General Hospital, Buffalo, NY 14203, USA.
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  • Paras N. Prasad,

    Corresponding author
    1. Institute for Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY 14260-4200, USA
    • Institute for Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY 14260-4200, USA
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  • Chong Cheng

    Corresponding author
    1. Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260-4200, USA
    • Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260-4200, USA
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Abstract

Well-defined tertiary amine-functionalized cationic polylactides (CPLAs) are synthesized by thiol-ene click functionalization of an allyl-functionalized polylactide, and utilized for the delivery of interleukin-8 (IL-8) siRNA via CPLA-IL-8 siRNA nanoplexes. The CPLAs possess remarkable hydrolytic degradability, and their cytotoxicity is relatively low. The CPLA-IL-8 siRNA nanoplexes can be readily taken up by prostate cancer cells, resulting in significant IL-8 gene silencing. It is found that the degradability and cytotoxicity of CPLAs, as well as the transfection efficiency of the CPLA-IL-8 siRNA nanoplexes, positively correlate with the amine mol% of CPLAs.

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