Fate of Intravenously Administered Gold Nanoparticles in Hair Follicles: Follicular Delivery, Pharmacokinetic Interpretation, and Excretion

Authors

  • Ivan M. Kempson,

    Corresponding author
    1. Institute of Physics, Academia Sinica, Taipei, 11529, Taiwan, Ian Wark Research Institute, University of South Australia, Mawson Lakes, S.A., 5095, Australia
    • Institute of Physics, Academia Sinica, Taipei, 11529, Taiwan, Ian Wark Research Institute, University of South Australia, Mawson Lakes, S.A., 5095, Australia.
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  • Chia-Chi Chien,

    1. Institute of Physics, Academia Sinica, Taipei, 11529, Taiwan, Ian Wark Research Institute, University of South Australia, Mawson Lakes, S.A., 5095, Australia
    2. Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan
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  • Chao-Yu Chung,

    1. National Synchrotron Radiation Research Center, 101 HsinAnn Rd, Hsinchu, 30076, Taiwan
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  • Yeukuang Hwu,

    1. Institute of Physics, Academia Sinica, Taipei, 11529, Taiwan, Ian Wark Research Institute, University of South Australia, Mawson Lakes, S.A., 5095, Australia
    2. Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan
    3. Institute of Optoelectronic Sciences, National Taiwan Ocean University, Keelung 20224, Taiwan
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  • David Paterson,

    1. Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria, 3168, Australia
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  • Martin D. de Jonge,

    1. Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria, 3168, Australia
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  • Daryl L. Howard

    1. Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria, 3168, Australia
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Abstract

Gold nanoparticles (GNPs) are intravenously administered to mice. Deposition at the pilosebacious unit and whiskers is visualized with X-ray fluorescence after 30 minutes and 14 days. After 30 minutes the dermal papilla, bulge region, and root sheath all contain NPs. GNPs are driven externally out from follicles, counteractive to transfollicular delivery. After 14 days, gold bands in hairs reflect pharmacokinetic profiles indicating blood concentration kinetics. Elimination rate constants infer half-lives from 3 hairs from an individual mouse within reasonable agreement (6.08, 7.15, and 8.66 hours). 3D reconstruction of NP distributions with confocal microscopy identifies aggregates within the medullary canal. Intermittent NP deposition continues randomly over the two week period demonstrating prolonged NP mobility in vivo. NPs are still retained at the hair bulb after 14 days. The observations further account for the excretory mechanisms of NPs and their behavior in the pilosebacous unit, and demonstrate monitoring pharmacokinetic behavior in individual animals.

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