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An Automated Process for Layer-by-Layer Assembly of Polyelectrolyte Multilayer Thin Films on Viable Cell Aggregates

Authors

  • Joseph M. Mets,

    1. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Atlanta, GA 30332, USA
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  • John T. Wilson,

    1. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Atlanta, GA 30332, USA
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  • Wanxing Cui,

    1. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Atlanta, GA 30332, USA
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  • Elliot L. Chaikof

    Corresponding author
    1. Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School and the Wyss Institute of Biologically Inspired Engineering of Harvard University, 110 Francis Street, Suite 5B, Boston, MA 02115, USA
    • Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School and the Wyss Institute of Biologically Inspired Engineering of Harvard University, 110 Francis Street, Suite 5B, Boston, MA 02115, USA.
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Abstract

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An automated process for modifying the surface of pancreatic islets grows uniform polyelectrolyte multilayer thin films, eliminating user variability associated with previous manual methods. Machine vision feedback allows for tight control of small fluid volumes, maintaining an islet microenvironment. This process is adaptable to other fragile micrometer-scale particle systems.

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