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Combination of Anti-Biofouling and Ion-Interaction by Click Chemistry for Endotoxin Selective Removal from Protein Solution

Authors

  • Jinshan Guo,

    1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China
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  • Fanbo Meng,

    Corresponding author
    1. The Cardiology Department of China-Japan Union, Hospital of Jilin University, No. 126 Xiantai Str., Changchun 130033, P. R. China, Tel & Fax: +86-13159757035
    • The Cardiology Department of China-Japan Union, Hospital of Jilin University, No. 126 Xiantai Str., Changchun 130033, P. R. China, Tel & Fax: +86-13159757035.
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  • Xiabin Jing,

    1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China
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  • Yubin Huang

    Corresponding author
    1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China
    • State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China
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Abstract

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A successful combination of anti-biofouling and ion-interaction for endotoxin (ET) selective removal from protein solutions is realized via the immobilization of two ordinary polymers, PEG and PLL, which have protein resistance and endotoxin adsorption properties, respectively, together onto azide-functional polystyrene (PS-N3) microspheres (MSs) using a surface click reaction. Toxic copper ions residu is successfully minimized by the addition of 2,2′-bipyridine (BiPy).

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