Image-Predicated Sorting of Adherent Cells Using Photopatterned Hydrogels

Authors

  • Joseph Kovac,

    1. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
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  • Ylaine Gerardin,

    1. Department of Systems Biology, Harvard University, Cambridge, MA 02138, USA
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  • Joel Voldman

    Corresponding author
    1. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    • Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
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Abstract

original image

Using photopatterned hydrogels and selective cell encapsulation, populations of adherent cells are examined using microscopy and sorted into viable sub-populations predicated on their imaged phenotypes. The inexpensive method utilizes commercial reagents and equipment available in many labs, making image-predicated cell sorting an accessible technique for a large number of individual labs.

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