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Keywords:

  • genetic screens;
  • hydrogels;
  • microscopy;
  • photopatterning;
  • sorting
Thumbnail image of graphical abstract

Using photopatterned hydrogels and selective cell encapsulation, populations of adherent cells are examined using microscopy and sorted into viable sub-populations predicated on their imaged phenotypes. The inexpensive method utilizes commercial reagents and equipment available in many labs, making image-predicated cell sorting an accessible technique for a large number of individual labs.