Nanowell-Trapped Charged Ligand-Bearing Nanoparticle Surfaces: A Novel Method of Enhancing Flow-Resistant Cell Adhesion

Authors

  • Phat L. Tran,

    1. Biomedical Engineering Graduate Interdisciplinary, Program and Department of Biomedical Engineering, The University of Arizona, Tucson, Arizona 85721, USA
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  • Jessica R. Gamboa,

    1. Biomedical Engineering Graduate Interdisciplinary, Program and Department of Biomedical Engineering, The University of Arizona, Tucson, Arizona 85721, USA
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  • Katherine E. McCracken,

    1. Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, Arizona 85721, USA
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  • Mark R. Riley,

    1. Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, Arizona 85721, USA
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  • Marvin J. Slepian,

    Corresponding author
    1. Biomedical Engineering Graduate Interdisciplinary, Program and Department of Biomedical Engineering, The University of Arizona, Tucson, Arizona 85721, USA
    2. Sarver Heart Center and Department of Medicine, College of Medicine, The University of Arizona, Tucson, Arizona 85721, USA
    • Biomedical Engineering Graduate Interdisciplinary, Program and Department of Biomedical Engineering, The University of Arizona, Tucson, Arizona 85721, USA.
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  • Jeong-Yeol Yoon

    Corresponding author
    1. Biomedical Engineering Graduate Interdisciplinary, Program and Department of Biomedical Engineering, The University of Arizona, Tucson, Arizona 85721, USA
    2. Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, Arizona 85721, USA
    • Biomedical Engineering Graduate Interdisciplinary, Program and Department of Biomedical Engineering, The University of Arizona, Tucson, Arizona 85721, USA.
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Abstract

Assuring cell adhesion to an underlying biomaterial surface is vital in implant device design and tissue engineering, particularly under circumstances where cells are subjected to potential detachment from overriding fluid flow. Cell–substrate adhesion is a highly regulated process involving the interplay of mechanical properties, surface topographic features, electrostatic charge, and biochemical mechanisms. At the nanoscale level, the physical properties of the underlying substrate are of particular importance in cell adhesion. Conventionally, natural, pro-adhesive, and often thrombogenic, protein biomaterials are frequently utilized to facilitate adhesion. In the present study, nanofabrication techniques are utilized to enhance the biological functionality of a synthetic polymer surface, polymethymethacrylate, with respect to cell adhesion. Specifically we examine the effect on cell adhesion of combining: 1. optimized surface texturing, 2. electrostatic charge and 3. cell adhesive ligands, uniquely assembled on the substrata surface, as an ensemble of nanoparticles trapped in nanowells. Our results reveal that the ensemble strategy leads to enhanced, more than simply additive, endothelial cell adhesion under both static and flow conditions. This strategy may be of particular utility for enhancing flow-resistant endothelialization of blood-contacting surfaces of cardiovascular devices subjected to flow-mediated shear.

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