A Versatile Approach to Engineering Biomolecule-Presenting Cellular Microenvironments

Authors

  • Philipp S. Lienemann,

    1. Department of Obstetrics, University Hospital Zurich, Schmelzbergstr. 12, 8091 Zurich, Switzerland
    2. Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 15, Bld AI 1109, 1015 Lausanne, Switzerland
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  • Maria Karlsson,

    1. Faculty of Biology and BIOSS Centre for Biological, Signalling Studies, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany
    2. Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, 4058 Basel, Switzerland
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  • Ana Sala,

    1. Department of Obstetrics, University Hospital Zurich, Schmelzbergstr. 12, 8091 Zurich, Switzerland
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  • Hanna M. Wischhusen,

    1. Faculty of Biology and BIOSS Centre for Biological, Signalling Studies, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany
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  • Franz E. Weber,

    1. Department of Cranio Maxillofacial Surgery, University Hospital Zurich, Frauenklikstrasse 24, 8091 Zurich, Switzerland
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  • Roland Zimmermann,

    1. Department of Obstetrics, University Hospital Zurich, Schmelzbergstr. 12, 8091 Zurich, Switzerland
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  • Wilfried Weber,

    1. Faculty of Biology and BIOSS Centre for Biological, Signalling Studies, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany
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  • Matthias P. Lutolf,

    1. Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 15, Bld AI 1109, 1015 Lausanne, Switzerland
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  • Martin Ehrbar

    Corresponding author
    1. Department of Obstetrics, University Hospital Zurich, Schmelzbergstr. 12, 8091 Zurich, Switzerland
    • Department of Obstetrics, University Hospital Zurich, Schmelzbergstr. 12, 8091 Zurich, Switzerland.
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Abstract

original image

By combining a novel protein-capture hydrogel with state-of-the-art mammalian recombinant protein production, cellular microenvironments are fabricated that locally instruct in-vitro cell behavior through selective presentation of the expressed proteins.

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