Phenotypic and Transcriptional Modulation of Human Pluripotent Stem Cells Induced by Nano/Microfabrication Materials

Authors

  • Ken-ichiro Kamei,

    Corresponding author
    1. Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan
    Current affiliation:
    1. These authors contributed equally to this work.
    • Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan
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  • Yoshikazu Hirai,

    1. Department of Micro Engineering, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto, 606-8501, Japan
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Momoko Yoshioka,

    1. Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan
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  • Yoshihide Makino,

    1. Department of Micro Engineering, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto, 606-8501, Japan
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  • Qinghua Yuan,

    1. Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan
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  • Minako Nakajima,

    1. Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan
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  • Yong Chen,

    1. Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan
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  • Osamu Tabata

    Corresponding author
    1. Department of Micro Engineering, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto, 606-8501, Japan
    • Department of Micro Engineering, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto, 606-8501, Japan.
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Abstract

original image

Microfabrication materials such as polydimethylsiloxane (PDMS) and photoresists (PRs) permit the creation of nano/microstructured substrates that enable the direct control of the cellular functions and phenotypes of human pluripotent stem cells (hPSCs). The multiple analyses of cellular phenotypes and gene expression demonstrate that microfabrication materials preferentially direct hPSCs towards differentiation.

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