Nanospiderwebs: Artificial 3D Extracellular Matrix from Nanofibers by Novel Clinical Grade Electrospinning for Stem Cell Delivery
Article first published online: 1 NOV 2012
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Healthcare Materials
Volume 2, Issue 5, pages 702–717, May, 2013
How to Cite
Alamein, M. A., Liu, Q., Stephens, S., Skabo, S., Warnke, F., Bourke, R., Heiner, P. and Warnke, P. H. (2013), Nanospiderwebs: Artificial 3D Extracellular Matrix from Nanofibers by Novel Clinical Grade Electrospinning for Stem Cell Delivery. Advanced Healthcare Materials, 2: 702–717. doi: 10.1002/adhm.201200287
- Issue published online: 2 MAY 2013
- Article first published online: 1 NOV 2012
- Manuscript Received: 17 AUG 2012
- good manufacturing practice;
- mesenchymal stem cells;
- tissue engineering
Novel clinical grade electrospinning methods could provide three-dimensional (3D) nanostructured biomaterials comprising of synthetic or natural biopolymer nanofibers. Such advanced materials could potentially mimic the natural extracellular matrix (ECM) accurately and may provide superior niche-like spaces on the subcellular scale for optimal stem-cell attachment and individual cell homing in regenerative therapies. The goal of this study was to design several novel “nanofibrous extracellular matrices” (NF-ECMs) with a natural mesh-like 3D architecture through a unique needle-free multi-jet electrospinning method in highly controlled manner to comply with good manufacturing practices (GMP) for the production of advanced healthcare materials for regenerative medicine, and to test cellular behavior of human mesenchymal stem cells (HMSCs) on these.
Biopolymers manufactured as 3D NF-ECM meshes under clinical grade GMP-like conditions show higher intrinsic cytobiocompatibility with superior cell integration and proliferation if compared to their 2D counterparts or a clinically-approved collagen membrane.