Three-Dimensional Human Tissue Chips Fabricated by Rapid and Automatic Inkjet Cell Printing

Authors

  • Michiya Matsusaki,

    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 Japan
    2. PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho Kawaguchi, Saitama, Japan
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  • Kayo Sakaue,

    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 Japan
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  • Koji Kadowaki,

    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 Japan
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  • Mitsuru Akashi

    Corresponding author
    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 Japan
    • Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 Japan.
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Abstract

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Rapid and automatic development of three-dimensional (3D) human micro-tissue chips which integrate over 400 micro-arrays of simplified multilayered structures is carried out using inkjet printing technology. Comprehensive high-throughput assays of liver functions using the simplified 3D liver structures revealed highest functions of a sandwich structure of endothelial cells and hepatocytes. These 3D-human tissue chips may enable “total-human tissue models” for tailor-made drug screening.

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