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Optically Traceable Solid Lipid Nanoparticles Loaded with siRNA and Paclitaxel for Synergistic Chemotherapy with In situ Imaging

Authors

  • Ki Hyun Bae,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseung-gu, Daejeon, 305-701, Republic of Korea
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Jeong Yu Lee,

    1. Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseung-gu, Daejeon, 305-701, Republic of Korea
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Soo Hyeon Lee,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseung-gu, Daejeon, 305-701, Republic of Korea
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  • Tae Gwan Park,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseung-gu, Daejeon, 305-701, Republic of Korea
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  • Yoon Sung Nam

    Corresponding author
    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseung-gu, Daejeon, 305-701, Republic of Korea
    2. Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseung-gu, Daejeon, 305-701, Republic of Korea
    3. KAIST Institute of NanoCentury (KINC) and BioCentury (KIB), Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseung-gu, Daejeon, 305-701, Republic of Korea
    • Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseung-gu, Daejeon, 305-701, Republic of Korea.
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Abstract

Here, we report quantum dot-incorporating solid lipid nanoparticles (SLNs) for anticancer theranostics with synergistic therapeutic effects of paclitaxel-siRNA combination. The natural components of a low-density lipoprotein (LDL) are reconstituted to produce LDL-mimetic SLNs having a stable core/shell nanostructure incorporating quantum dots and paclitaxel within the lipid shell while anionic siRNA molecules are electrostatically complexed with the outer surface of SLNs. The produced SLN/siRNA complexes efficiently deliver both of paclitaxel and Bcl-2 targeted siRNA into human lung carcinoma cells and exhibit synergistic anticancer activities by triggering caspase-mediated apoptosis as determined by median effect plot analysis. Moreover, the strong fluorescence from quantum dots within SLNs enables in situ visualization of intracellular translocation of SLNs into cancer cells. Our study suggests that LDL-mimetic SLNs can be utilized as a multifunctional and optically traceable nanocarrier for efficient anticancer theranostics.

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