Assembly of Discrete Collagen–Chitosan Microenvironments into Multiphase Tissue Constructs

Authors

  • David J. Caldwell,

    1. Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave. Ann Arbor, MI 48109, USA, Tel: 734-764-8313; Fax: 734-647-4834
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  • Rameshwar R. Rao,

    1. Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave. Ann Arbor, MI 48109, USA, Tel: 734-764-8313; Fax: 734-647-4834
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  • Jan P. Stegemann

    Corresponding author
    1. Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave. Ann Arbor, MI 48109, USA, Tel: 734-764-8313; Fax: 734-647-4834
    • Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave. Ann Arbor, MI 48109, USA, Tel: 734-764-8313; Fax: 734-647-4834.
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Abstract

original image

Modular assembly of protein–polysaccharide microenvironments into 3D macroscale tissue constructs is reported. Rapid and simple centrifugation and vacuum molding methods are used to create cohesive multiphase constructs with prescribed geometries. Human fibroblasts are shown to survive in the microenvironments and in the macroscale constructs. Control of the spatial organization in engineered tissues is a key to recreating the complex tissue architectures needed for regenerative therapies.

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