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Manipulating Magnetic 3D Spheroids in Hanging Drops for Applications in Tissue Engineering and Drug Screening

Authors

  • Vincent H. B. Ho,

    Corresponding author
    1. Molecular Engineering Laboratory, A*STAR, Proteos #03-13, 61 Biopolis Drive, Singapore 138673, Singapore
    • Vincent H. B. Ho, Molecular Engineering Laboratory, A*STAR, Proteos #03-13, 61 Biopolis Drive, Singapore 138673, Singapore

      Joachim S.C. Loo, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.

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  • Wei Mei Guo,

    1. Molecular Engineering Laboratory, A*STAR, Proteos #03-13, 61 Biopolis Drive, Singapore 138673, Singapore
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Charlotte L. Huang,

    1. School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Shu Fen Ho,

    1. Molecular Engineering Laboratory, A*STAR, Proteos #03-13, 61 Biopolis Drive, Singapore 138673, Singapore
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  • Su Yin Chaw,

    1. School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
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  • Ern Yu Tan,

    1. Department of General Surgery, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, Singapore 308433, Singapore
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  • Kee Woei Ng,

    1. School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
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  • Joachim S.C. Loo

    Corresponding author
    1. School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
    • Vincent H. B. Ho, Molecular Engineering Laboratory, A*STAR, Proteos #03-13, 61 Biopolis Drive, Singapore 138673, Singapore

      Joachim S.C. Loo, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.

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  • This work is dedicated to the late Professor Ma Jan who was a mentor to many of us.

Abstract

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Magnetic spheroid manipulation can be carried out in hanging drops to generate distinctly structured heterotypic microtissues through sequential addition of cells or spheroid to homotypic spheroids. These spheroids can also be incorporated in a droplet-based assay to screen for therapeutic efficacy in prolonged studies. This simple and versatile technique can offer potential benefits in tissue engineering and drug screening applications.

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