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Two-Photon Ratiometric Fluorescent Mapping of Intracellular Transport Pathways of pH-Responsive Block Copolymer Micellar Nanocarriers

Authors

  • Tao Liu,

    1. CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China
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  • Jinming Hu,

    1. CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China
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  • Zhenyu Jin,

    1. CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China
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  • Fan Jin,

    Corresponding author
    1. CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China
    • CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China.

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  • Shiyong Liu

    Corresponding author
    1. CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China
    • CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China.

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Abstract

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Ultrasensitive ratiometric fluorescent pH probes based on dual dye-labeled pH-responsive diblock copolymer micellar scaffold are constructed. The pH-sensitive emitting nature of BTPE dyes and emission turn-on of CMA moieties triggered by pH-actuated micelle-to-unimer of diblock scaffold synergistically contribute to the observed ≈250-fold changes of BTPE/CMA emission intensity ratios in the whole pH range. Two-photon ratiometric fluorescent pH mapping of intracellular gradients subjected by pH-responsive micellar nanoparticles in their endocytic pathway has been thus achieved.

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