Bio-Origami Hydrogel Scaffolds Composed of Photocrosslinked PEG Bilayers

Authors

  • Mustapha Jamal,

    1. Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 125 Maryland Hall, Baltimore, Maryland 21218, USA
    2. Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, Maryland 21218, USA
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  • Sachin S. Kadam,

    1. Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 125 Maryland Hall, Baltimore, Maryland 21218, USA
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  • Rui Xiao,

    1. Department of Mechanical Engineering, Johns Hopkins University, 125 Latrobe Hall, Baltimore, Maryland 21218, USA
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  • Faraz Jivan,

    1. Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 125 Maryland Hall, Baltimore, Maryland 21218, USA
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  • Tzia-Ming Onn,

    1. Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 125 Maryland Hall, Baltimore, Maryland 21218, USA
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  • Rohan Fernandes,

    1. Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 125 Maryland Hall, Baltimore, Maryland 21218, USA
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  • Thao D. Nguyen,

    1. Department of Mechanical Engineering, Johns Hopkins University, 125 Latrobe Hall, Baltimore, Maryland 21218, USA
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  • David H. Gracias

    Corresponding author
    1. Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 125 Maryland Hall, Baltimore, Maryland 21218, USA
    2. Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    3. Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA
    • Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 125 Maryland Hall, Baltimore, Maryland 21218, USA.

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Abstract

We describe the self-folding of photopatterned poly (ethylene glycol) (PEG)-based hydrogel bilayers into curved and anatomically relevant micrometer-scale geometries. The PEG bilayers consist of two different molecular weights (MWs) and are photocrosslinked en masse using conventional photolithography. Self-folding is driven by differential swelling of the two PEG bilayers in aqueous solutions. We characterize the self-folding of PEG bilayers of varying composition and develop a finite element model which predicts radii of curvature that are in good agreement with empirical results. Since we envision the utility of bio-origami in tissue engineering, we photoencapsulate insulin secreting β-TC-6 cells within PEG bilayers and subsequently self-fold them into cylindrical hydrogels of different radii. Calcein AM staining and ELISA measurements are used to monitor cell proliferation and insulin production respectively, and the results indicate cell viability and robust insulin production for over eight weeks in culture.

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