Enhanced Osteogenesis by a Biomimic Pseudo-Periosteum-Involved Tissue Engineering Strategy

Authors

  • Xuetao Shi,

    1. WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan E-mail: mrshixuetao@gmail.com
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  • Song Chen,

    1. Biomaterials Unit, National Institute for materials Science, Tsukuba, Japan
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  • Yihua Zhao,

    1. Department of Chemistry, Hong Kong University of Science and Technology, Hong Kong, China
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  • Chen Lai,

    1. Biomedical Engineering Research Center, Shenzhen Institution of Beijing University, Shenzhen, China
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  • Hongkai Wu

    Corresponding author
    1. WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan E-mail: mrshixuetao@gmail.com
    2. Department of Chemistry, Hong Kong University of Science and Technology, Hong Kong, China
    3. Division of Biomedical Engineering, Hong Kong University of Science and Technology, Hong Kong, China
    • WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan E-mail: mrshixuetao@gmail.com.
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Abstract

Elaborating a bone replacement using tissue-engineering strategies for bone repair seems to be a promising remedy. However, previous platforms are limited in constructing three-dimensional porous scaffolds and neglected the critical importance of periosteum (a pivotal source of osteogenic cells for bone regeneration). We report here an innovative method using the periosteum as a template to replicate its exquisite morphologies onto the surfaces of biomaterials. The precise topographic cues (grooved micropatterns) on the surface of collagen membrane inherited from the periosteum effectively directed cell alignment as the way of natural periosteum. Besides, we placed the stem-cell and endothelial-cell-laden collagen membrane (pseudo-periosteum) onto a three-dimensional porous scaffold. The pseudo-periosteum-covered scaffolds showed remarkable osteogenesis when compared with the pseudo-periosteum-free scaffolds, indicating the significant importance of pseudo-periosteum on bone regeneration. This study gives a novel concept for the construction of bone tissue engineering scaffold and may provide new insight for periosteum research.

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