Get access

Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces

Authors

  • Zheng Qu,

    1. Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School and the Wyss Institute of Biologically Inspired, Engineering of Harvard University, Boston, MA 02115, USA
    2. Coulter Department of Biomedical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
    Search for more papers by this author
  • Venkat Krishnamurthy,

    1. Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School and the Wyss Institute of Biologically Inspired, Engineering of Harvard University, Boston, MA 02115, USA
    Search for more papers by this author
  • Carolyn A. Haller,

    1. Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School and the Wyss Institute of Biologically Inspired, Engineering of Harvard University, Boston, MA 02115, USA
    Search for more papers by this author
  • Brent M. Dorr,

    1. Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Search for more papers by this author
  • Ulla M. Marzec,

    1. Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA
    Search for more papers by this author
  • Sawan Hurst,

    1. Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA
    Search for more papers by this author
  • Monica T. Hinds,

    1. Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA
    Search for more papers by this author
  • Stephen R. Hanson,

    1. Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA
    Search for more papers by this author
  • David R. Liu,

    1. Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Search for more papers by this author
  • Elliot L. Chaikof

    Corresponding author
    1. Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School and the Wyss Institute of Biologically Inspired, Engineering of Harvard University, Boston, MA 02115, USA
    2. Coulter Department of Biomedical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
    • Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School and the Wyss Institute of Biologically Inspired, Engineering of Harvard University, Boston, MA 02115, USA.

    Search for more papers by this author

Abstract

original image

Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

Ancillary