Cellulose-Based Diagnostic Devices for Diagnosing Serotype-2 Dengue Fever in Human Serum

Authors

  • Hsi-Kai Wang,

    1. Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan
    2. Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 128, Taiwan
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  • Cheng-Han Tsai,

    1. Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan
    2. Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan
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  • Kuan-Hung Chen,

    1. Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan
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  • Chung-Tao Tang,

    1. Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 128, Taiwan
    2. Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan
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  • Jiun-Shyang Leou,

    1. Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 128, Taiwan
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  • Pi-Chun Li,

    1. Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 128, Taiwan
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  • Yin-Liang Tang,

    1. Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 128, Taiwan
    2. Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan
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  • Hsyue-Jen Hsieh,

    1. Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan
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  • Han-Chung Wu,

    Corresponding author
    1. Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 128, Taiwan
    • Han-Chung Wu, Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 128, Taiwan.

      Chao-Min Cheng, Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan

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  • Chao-Min Cheng

    Corresponding author
    1. Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan
    2. Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 128, Taiwan
    • Han-Chung Wu, Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 128, Taiwan.

      Chao-Min Cheng, Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan

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Abstract

Here, two types of cellulose-based in vitro diagnostic devices are demonstrated for the diagnosis of dengue virus infection in both buffer system and human serum: 1) paper-based ELISA for providing the semiquantitative information of the disease activity of serotype-2 dengue fever to healthcare persons (i.e., monitoring the disease activity with a specific serotype in single patients); 2) lateral flow immunoassays to screen for infection with serotype-2 dengue fever (i.e., rapid YES or NO diagnosis prepared for large populations, in terms of global public health). Paper-based ELISA (specific to serotype-2 dengue fever), which builds off of our previous studies and a revised previous ELISA procedure, owns multiple advantages: 1) high sensitivity (about 40 times higher than the current ELISA-based approaches, due to our therapeutic-based monoclonal antibody) and specificity (specific to dengue virus serotype-2 nonstructural protein-1 antigens); 2) tiny amount of sample and reagent used for single tests; 3) short operating duration (i.e., rapid diagnostic device); and, 4) inexpensiveness (appropriate for use in all developing and underdeveloped nations of the world). Due to the higher sensitivity and shorter operating duration of paper-based ELISA (compared with conventional ELISA, and lateral flow immunoassays also performed in this study), this study has not only been able to perform the diagnosis of dengue virus serotype-2 nonstructural protein-1 antigens in both buffer system and human serum but also to evaluate dengue virus serotype-2 envelope proteins in the buffer system, thus successfully achieving the first such use of these proteins as the target antigen for the development of diagnostic tools. These results provide a more comprehensive understanding for the genesis of dengue fever diagnostic tools (through antibody-antigen recognition).

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