Rational Design of Phosphorescent Chemodosimeter for Reaction-Based One- and Two-Photon and Time-Resolved Luminescent Imaging of Biothiols in Living Cells

Authors

  • Wenjuan Xu,

    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    Search for more papers by this author
  • Xin Zhao,

    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    Search for more papers by this author
  • Wen Lv,

    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    Search for more papers by this author
  • Huiran Yang,

    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    Search for more papers by this author
  • Shujuan Liu,

    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    Search for more papers by this author
  • Hua Liang,

    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    Search for more papers by this author
  • Zhenzhen Tu,

    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    Search for more papers by this author
  • Hang Xu,

    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    Search for more papers by this author
  • Weili Qiao,

    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    Search for more papers by this author
  • Qiang Zhao,

    Corresponding author
    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    Search for more papers by this author
  • Wei Huang

    Corresponding author
    1. Center for Phosphorescent Optoelectronics (CPO), Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, P. R. China
    2. Jiangsu-Singapore Joint Research Center for Organic/Bio Electronics & Information Displays, and Institute of Advanced Materials, Nanjing University of Technology, Nanjing, P. R. China
    Search for more papers by this author

Abstract

A selective phosphorescent biothiols probe is synthesized based on Ir(III) complex 1, which has 2,2′-biquinoline as the N^N ligand for realizing the satisfied two-photon absorption cross-section and two-functionalized 2-phenylpyridine ligands with an α,β-unsaturated ketone moiety as the thiol reaction site. The one- and two-photon optical properties of 1 are investigated through UV–vis absorption spectrum and photoluminescence spectrum. This Ir(III) complex can act as an excellent one- and two-photon excited “OFF–ON” phosphorescent probe for biothiols based on the 1,4-addition of biothiol to α,β-unsaturated ketones. Moreover, one- and two-photon-induced luminescent imagings of biothiols in living cells are also realized. Furthermore, the experiments of time-resolved photoluminescence technique and fluorescence lifetime imaging microscopy demonstrate that 1 is able to detect biothiols in the presence of strong background fluorescence. In addition, probe 1 is adsorbed into the shell of mesoporous silica nanoparticles with core–shell structure to form a nanoprobe, which can realize the ratiometric detection of biothiols in absolute water solution and living cells based on two phosphorescent signals.

Ancillary