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Nanofibers Implant Functionalized by Neural Growth Factor as a Strategy to Innervate a Bioengineered Tooth

Authors

  • Sandy Eap,

    1. Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine, Faculté de Médecine, Strasbourg Cedex, France
    2. Université de Strasbourg Faculté de Chirurgie Dentaire, Strasbourg, France
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  • Thibault Bécavin,

    1. Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine, Faculté de Médecine, Strasbourg Cedex, France
    2. Université de Strasbourg Faculté de Chirurgie Dentaire, Strasbourg, France
    3. Université Lille Nord de France Faculté de Chirurgie Dentaire INSERM UMR 1008 “Controlled Drug Delivery Systems and Biomaterials”, Lille, France
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  • Laetitia Keller,

    1. Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine, Faculté de Médecine, Strasbourg Cedex, France
    2. Université de Strasbourg Faculté de Chirurgie Dentaire, Strasbourg, France
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  • Tunay Kökten,

    1. Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine, Faculté de Médecine, Strasbourg Cedex, France
    2. Université de Strasbourg Faculté de Chirurgie Dentaire, Strasbourg, France
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  • Florence Fioretti,

    1. Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine, Faculté de Médecine, Strasbourg Cedex, France
    2. Université de Strasbourg Faculté de Chirurgie Dentaire, Strasbourg, France
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  • Jean-Luc Weickert,

    1. Institut de Génétique et de Biologie Moléculaire et Cellulaire Service de Microscopie Electronique, France
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  • Etienne Deveaux,

    1. Université Lille Nord de France Faculté de Chirurgie Dentaire INSERM UMR 1008 “Controlled Drug Delivery Systems and Biomaterials”, Lille, France
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  • Nadia Benkirane-Jessel,

    Corresponding author
    1. Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine, Faculté de Médecine, Strasbourg Cedex, France
    2. Université de Strasbourg Faculté de Chirurgie Dentaire, Strasbourg, France
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  • Sabine Kuchler-Bopp

    1. Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine, Faculté de Médecine, Strasbourg Cedex, France
    2. Université de Strasbourg Faculté de Chirurgie Dentaire, Strasbourg, France
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Abstract

Current strategies for jaw reconstruction require multiple procedures, to repair the bone defect, to offer sufficient support, and to place the tooth implant. The entire procedure can be painful and time-consuming, and the desired functional repair can be achieved only when both steps are successful. The ability to engineer combined tooth and bone constructs, which would grow in a coordinated fashion with the surrounding tissues, could potentially improve the clinical outcomes and also reduce patient suffering. A unique nanofibrous and active implant for bone–tooth unit regeneration and also the innervation of this bioengineered tooth are demonstrated. A nanofibrous polycaprolactone membrane is functionalized with neural growth factor, along with dental germ, and tooth innervation follows. Such innervation allows complete functionality and tissue homeostasis of the tooth, such as dentinal sensitivity, odontoblast function, masticatory forces, and blood flow.

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