• negative photoresists;
  • polydimethylsiloxane;
  • pluripotent stem cells;
  • cellular phenotypes;
  • gene expression
Thumbnail image of graphical abstract

Polymer-based microfabrication materials such as polydimethylsiloxane (PDMS) and photoresists (PRs) permit the creation of nano/microstructured substrates that enable the direct control of the cellular functions and phenotypes of human pluripotent stem cells (hPSCs), but their biocompatibility and effects on hPSCs are poorly understood. On page 287, Osamu Tabata, Kenichiro Kamei, and co-workers reveal that the tested materials preferentially direct hPSCs towards differentiation. The image depicts how PRs (yellow), PDMS (light blue) and glass (white) influence the capability of hPSC differentiation into various tissues, such as muscle, heart and neurons.