Tissue Engineering: Millimeter-Sized Neural Building Blocks for 3D Heterogeneous Neural Network Assembly (Adv. Healthcare Mater. 12/2013)

Authors

  • Midori Kato-Negishi,

    1. Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
    2. Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 4-6-1, Komaba, Meguro-ku, Tokyo 153-8505, Japan
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  • Yuya Morimoto,

    1. Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
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  • Hiroaki Onoe,

    1. Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
    2. Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 4-6-1, Komaba, Meguro-ku, Tokyo 153-8505, Japan
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  • Shoji Takeuchi

    Corresponding author
    1. Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
    2. Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 4-6-1, Komaba, Meguro-ku, Tokyo 153-8505, Japan
    • Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.

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Abstract

original image

A millimeter-sized neural component, named neural building block (NBB), is formed by using massive amount of neuronal spheroids from a single specific brain region. By assembling different NBBs, Shoji Takeuchi and co-workers show, on page 1564, how 3D heterogeneous macroscopic neural tissue can be fabricated without any scaffold materials. This method offers high cell density cultures, and neurons in this system show complex millimeter-scale axonal extensions into 3D spatial areas.

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