Endotoxin Removal by Magnetic Separation-Based Blood Purification (pages 829–835)
Inge K. Herrmann, Martin Urner, Samuel Graf, Christoph M. Schumacher, Birgit Roth-Z'graggen, Melanie Hasler, Wendelin J. Stark and Beatrice Beck-Schimmer
Version of Record online: 6 DEC 2012 | DOI: 10.1002/adhm.201200358
Playing a central role in the development of gram-negative sepsis, endotoxins are attractive therapeutic targets. However, their direct removal from protein-rich fluids remains challenging. This article presents a magnetic separation-based approach using polymyxin B-functionalized metal nanomagnets to eliminate endotoxin from human blood in vitro. Functional assays show an attenuated inflammatory response from human endothelial cells and less chemotactic activity in purified samples.