Amperometric biosensors produced by immobilization of redox enzymes at polythiophene-modified electrode surfaces

Authors

  • Markus Hiller,

    1. Institut für Organische Chemie Universität Würzburg
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  • Christine Kranz,

    1. Lehrstuhl für Allgemeine Chemie und Biochemie Technische Universität München Vöttingerstrasse 40 D-85350 Freising-Weihenstephan (Germany)
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  • Johanna Huber,

    1. Lehrstuhl für Allgemeine Chemie und Biochemie Technische Universität München Vöttingerstrasse 40 D-85350 Freising-Weihenstephan (Germany)
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  • Prof. Peter Bäuerle,

    Corresponding author
    1. Institut für Organische Chemie Universität Würzburg
    • Institut für Organische Chemie Universität Würzburg
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  • Dr. Wolfgang Schuhmann

    Corresponding author
    1. Lehrstuhl für Allgemeine Chemie und Biochemie Technische Universität München Vöttingerstrasse 40 D-85350 Freising-Weihenstephan (Germany)
    • Institut für Organische Chemie Universität Würzburg
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Abstract

The use of polythiophene films for the immobilization of enzymes at electrodes is shown to have advantages over other conducting polymer films. Two methods by which the enzymes can be immobilized—entrapment and covalent immobilization at the surface—are described and compared. The improved stability of polythiophene against oxidation in ambient environments is expected to enable the construction of biosensors with improved sensor characteristics and lifetime.

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