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The microscopic structure of skeletonized films of cadmium arachidate has been studied by atomic force microscopy (AFM)—skeletonization is the removal of free acid from a mixed Langmuir–Blodgett (LB) films and the AFM methods used are detailed, AFM images and results from a two-dimensional fast Fourier transform analysis are discussed, and factors affecting the overall cadmium arachidate content of the transferred film are considered.