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Coupling of an induced fit enzyme to polydiacetylene thin films: Colorimetric detection of glucose

Authors

  • Dr. Quan Cheng,

    1. Department of Chemistry, University of California Berkeley, CA 94720 (USA) and Materials Sciences Division Lawrence Berkeley National Laboratory Berkeley, CA 94720 (USA)
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  • Prof. Raymond C. Stevens

    1. Department of Chemistry, University of California Berkeley, CA 94720 (USA) and Materials Sciences Division Lawrence Berkeley National Laboratory Berkeley, CA 94720 (USA)
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  • This work was supported by the Director, Office of Energy Research, Office of Basic Energy Sciences. Division of Material Sciences, and the Division of Energy Biosciences of the U.S. Department of Energy, Contract No. DE-AC03-76SF0098. The work was also supported by the NSF Young Investigator Award (RCS) and the Beckman Foundation Young Investigator Award (RCS). We thank Professor Robert Fletterick for suggesting the hexokinase system. Nathanial David for critical reading of the manuscript, and Mark Alper for continued encouragement of this research program.

Abstract

A new biosensor design is demonstrated, in which a protein conformational change is used as a trigger mechanism for a low-cost, solid-state sensor (see also the cover). The figure illustrates the principle: an enzyme (hexokinase, large white shape) immobilized on a lipid polydiacetylene (PDA) layer undergoes a conformational change when glucose (dark shape) binds to it. The resulting stress in the PDA layer produces a color change.

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