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Advanced Materials

Cell-Responsive Synthetic Hydrogels

Authors

  • M.P. Lutolf,

    1. Institute for Biomedical Engineering and Department of Materials Science, Swiss Federal Institute of Technology Zurich (ETHZ) and University of Zurich, Moussonstrasse 18, CH-8044 Zurich, Switzerland
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  • G.P. Raeber,

    1. Institute for Biomedical Engineering and Department of Materials Science, Swiss Federal Institute of Technology Zurich (ETHZ) and University of Zurich, Moussonstrasse 18, CH-8044 Zurich, Switzerland
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  • A.H. Zisch,

    1. Institute for Biomedical Engineering and Department of Materials Science, Swiss Federal Institute of Technology Zurich (ETHZ) and University of Zurich, Moussonstrasse 18, CH-8044 Zurich, Switzerland
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  • N. Tirelli,

    1. Institute for Biomedical Engineering and Department of Materials Science, Swiss Federal Institute of Technology Zurich (ETHZ) and University of Zurich, Moussonstrasse 18, CH-8044 Zurich, Switzerland
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  • J.A. Hubbell

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  • This work was funded by a grant from the Swiss National Science Foundation and the European Union Fifth Framework. MMP-1 was a generous gift from Dr. G. B. Fields, Florida Atlantic University, Boca Raton, FL (USA) and Dr. H. Nagase, Imperial College of Science, Technology and Medicine, London (UK). Polyurethane sponges were a generous gift from Dr. D. Bezuidenhout and Dr. Zilla, Cape Heart Center, University of Cape Town, South Africa. We thank Dr. M. Höchli and Dr. T. Bächi of the Electron Microscopy Laboratory (EMZ) at the University of Zurich for help with CLSM.

Abstract

Synthetic biomaterial matrices have been formed in the presence of cells by a Michael-type addition reaction between a difunctional protease substrate peptide, a monofunctional cell adhesion peptide, and a tetrafunctional poly(ethylene glycol). These materials locally degrade in response to cell-surface proteases, allowing cells to create paths for 3D cell migration (see Figure).

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