Financial supports from the National Science Foundation (DMR 0 097 611), National Institutes of Health (GM62958-01), and the Office of Naval Research are gratefully acknowledged.
Optically Amplified RNA–Protein Detection Methods Using Light-Harvesting Conjugated Polymers†
Article first published online: 1 SEP 2003
Copyright © 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 15, Issue 17, pages 1425–1428, September, 2003
How to Cite
Wang, S. and Bazan, G.C. (2003), Optically Amplified RNA–Protein Detection Methods Using Light-Harvesting Conjugated Polymers. Adv. Mater., 15: 1425–1428. doi: 10.1002/adma.200305099
- Issue published online: 1 SEP 2003
- Article first published online: 1 SEP 2003
- Manuscript Accepted: 21 MAY 2003
- Manuscript Received: 12 MAR 2003
- Conjugated polymers;
- Optical amplification
A sensor is described that takes advantage of highly specific RNA–protein interactions and the light-harvesting properties of conjugated polymers. Coordination between a cationic conjugated polymer (CP+) and a negatively charged protein–RNA complex results in efficient Förster resonance energy transfer (FRET) from CP+ to a protein-bound chromophore (see Figure and inside cover). This sensor was used to detect the transactivation responsive RNA sequence of HIV-1.