Communication

A Novel Hierarchical Nanozeolite Composite as Sorbent for Protein Separation in Immobilized Metal-Ion Affinity Chromatography†

  1. F. Xu,
  2. Y. Wang,
  3. X. Wang,
  4. Y. Zhang,
  5. Y. Tang,
  6. P. Yang

Article first published online: 23 OCT 2003

DOI: 10.1002/adma.200305287

Issue

Advanced Materials

Advanced Materials

Volume 15, Issue 20, pages 1751–1753, October, 2003

Additional Information

How to Cite

Xu, F., Wang, Y., Wang, X., Zhang, Y., Tang, Y. and Yang, P. (2003), A Novel Hierarchical Nanozeolite Composite as Sorbent for Protein Separation in Immobilized Metal-Ion Affinity Chromatography. Advanced Materials, 15: 1751–1753. doi: 10.1002/adma.200305287

Author Information

  1. Department of Chemistry, Fudan University, Shanghai 200433, China

  1. †

    This research is supported by the National Basic Research Priorities Programme (Grant Nos. 2001CB510202 and 2000077500), the National HighTec Research Developing Programme (2001AA233031), the Shanghai Science & Technology Committee (00JC14014, 0249nm028), NSFC (20273016, 20233030), and the Key Project of Science and Technology of the of Minister of Education.

Publication History

  1. Issue published online: 23 OCT 2003
  2. Article first published online: 23 OCT 2003
  3. Manuscript Accepted: 27 JUN 2003
  4. Manuscript Received: 21 APR 2003

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Keywords:

  • Chromatography;
  • Protein separation;
  • Zeolites

A new hierarchical porous zeolite–diatomite composite (see Figure inset) of excellent mechanical and chemical stability, after immobilization of Co2+ by an ion-exchange process, was utilized as a packing sorbent for the separation of peptides and proteins containing histidine clusters. The Figure shows the chromatographic profile for two peptides separated using immobilized metal-ion affinity chromatography.

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