A Novel Hierarchical Nanozeolite Composite as Sorbent for Protein Separation in Immobilized Metal-Ion Affinity Chromatography


  • This research is supported by the National Basic Research Priorities Programme (Grant Nos. 2001CB510202 and 2000077500), the National HighTec Research Developing Programme (2001AA233031), the Shanghai Science & Technology Committee (00JC14014, 0249nm028), NSFC (20273016, 20233030), and the Key Project of Science and Technology of the of Minister of Education.


A new hierarchical porous zeolite–diatomite composite (see Figure inset) of excellent mechanical and chemical stability, after immobilization of Co2+ by an ion-exchange process, was utilized as a packing sorbent for the separation of peptides and proteins containing histidine clusters. The Figure shows the chromatographic profile for two peptides separated using immobilized metal-ion affinity chromatography.