Alignment of Long DNA Molecules on Templates Generated via Dip-Pen Nanolithography


  • This work was supported by the Bindley Bioscience Center (BBC) at Purdue University. The authors acknowledge experimental help from Dr. Richard Haash (UIUC) to carry out the XPS characterization. All XPS experiments were performed at the Center for Microanalysis of Materials, UIUC, which is partially supported by U.S. Department of Energy under grant DEFG02-91-ER45 439.


A methodology to place and stretch long DNA molecules by coupling dip-pen Nanolithography with molecular combing is presented. Templates were fabricated on SiOx surfaces using a positively charged polymer, poly(allylamine hydrochloride). Upon molecular combing, DNA molecules elongate on the surface (for example, see Figure) and are localized by the presence of the charged lithographically defined structures.