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Advanced Materials

Enzyme Immobilization on Porous Silicon Surfaces

Authors


  • This work was performed under the auspices of the U. S. Department of Energy by University of California Lawrence Livermore National Laboratory under contract No. W-7405-Eng-48. The authors thank J. Del Eckels of LLNL for help with the flow cell system design and fabrication.

Abstract

β-Glucuronidase enzymes have been attached to a porous silicon surface through a direct silicon–carbon bond based linking system (see Figure). The attached enzymes display high activity and the photoluminescent (PL) properties and surface stability of the porous silicon are retained. Quenching of the PL is observed upon enzymatic breakdown of the substrate, leading to the possibility of a new class of chemical and biological sensors.

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