Dynamic Addressing of a Surface Pattern by a Stimuli-Responsive Fusion Protein


  • This research was supported in part by a grant from the National Institutes of Health (R01-GM-61232) to A.C. We also thank David Huston from Baylor College of Medicine in Dallas, TX, for generously providing the anti-thioredoxin monoclonal antibody. The AFM instrumentation was acquired through a multi-investigator research instrumentation award from the National Science Foundation (NSF-DBI-96-04785).


Thermodynamically reversible addressing of proteins (TRAP) is demonstrated. A stimuli-responsive elastin-like polypeptide fused to thioredoxin is used to reversibly address the fusion protein onto a chemically micropatterned surface template by switching the interaction between the surface and the stimuli- responsive polypeptide (see Figure).