This research was supported in part by a grant from the National Institutes of Health (R01-GM-61232) to A.C. We also thank David Huston from Baylor College of Medicine in Dallas, TX, for generously providing the anti-thioredoxin monoclonal antibody. The AFM instrumentation was acquired through a multi-investigator research instrumentation award from the National Science Foundation (NSF-DBI-96-04785).
Dynamic Addressing of a Surface Pattern by a Stimuli-Responsive Fusion Protein†
Article first published online: 13 FEB 2003
© 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 15, Issue 3, pages 248–251, February, 2003
How to Cite
Frey, W., Meyer, D.E. and Chilkoti, A. (2003), Dynamic Addressing of a Surface Pattern by a Stimuli-Responsive Fusion Protein. Adv. Mater., 15: 248–251. doi: 10.1002/adma.200390058
- Issue published online: 13 FEB 2003
- Article first published online: 13 FEB 2003
- Manuscript Accepted: 18 NOV 2002
- Manuscript Received: 9 SEP 2002
- Proteins, thermodynamically reversible addressing of - (TRAP)
Thermodynamically reversible addressing of proteins (TRAP) is demonstrated. A stimuli-responsive elastin-like polypeptide fused to thioredoxin is used to reversibly address the fusion protein onto a chemically micropatterned surface template by switching the interaction between the surface and the stimuli- responsive polypeptide (see Figure).