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Flow-Through-Type DNA Array Based on Ideally Ordered Anodic Porous Alumina Substrate

Authors

  • F. Matsumoto,

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  • K. Nishio,

    1. Kanagawa Academy of Science and Technology, 5-4-30 Nishi Hashimoto, Sagamihara, Kanagawa 229-1131, Japan
    2. Current address: Department of Applied Chemistry, School of Engineering, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachioji, Tokyo 192-0397, Japan.
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  • H. Masuda

    1. Kanagawa Academy of Science and Technology, 5-4-30 Nishi Hashimoto, Sagamihara, Kanagawa 229-1131, Japan
    2. Current address: Department of Applied Chemistry, School of Engineering, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachioji, Tokyo 192-0397, Japan.
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  • This work was partially supported by a Grant-in-Aid for Encouragement of Young Scientists (No. 15750130) for F. Matsumoto from the Japan Society for the Promotion of Science

Abstract

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A flow-through-type DNA array fabricated from an ideally ordered anodic porous alumina substrate is reported. Single-stranded probe DNA was affixed to the walls of the sub-micrometer-diameter holes, and fluorescently labelled target DNA was passed through the holes in the substrate. Fluorescence microscopy revealed an ordered array of fluorescent spots after the hybridization (see Figure), demonstrating the applicability of these porous alumina substrates to fabrication of high-density DNA arrays.

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