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Biologically Active Protein Gradients via Microstamping


  • The authors thank Ma Yuexia and Shu Wang for supplying the PC12 cells, Doreen Lai for assistance in TOF-SIMS analysis, and the Agency for Science, Technology and Research, Singapore for funding support (Grant No. 0221070014).


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Protein gradients on substrates are prepared using a microstamping technique. An Au pattern is transferred from a patterned silicon stamp to a polymeric substrate, and exposed polymer regions are then selectively activated for protein immobilization. The protein gradient is achieved by varying the spacing between the exposed polymer regions (see Figure).

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