The authors thank N. L. Jeon, H. Baskaran, and J. Jacot for helpful discussions; P. Mak and the Boston University Photonics Center for access and support for microfabrication facilities; and D. E. Discher for support and use of atomic force microscopes in the Biophysical Engineering Lab at the University of Pennsylvania. JYW acknowledges financial support from the Whitaker Foundation (RG-98–0506; TF 02–0026), NSF CAREER Award (BES-9985338), NIH grant (R01 HL072900–01 A1) and a Clare Boothe Luce Professorship from the Henry Luce Foundation.
Photopolymerization in Microfluidic Gradient Generators: Microscale Control of Substrate Compliance to Manipulate Cell Response†
Article first published online: 16 DEC 2004
Copyright © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 16, Issue 23-24, pages 2133–2137, December, 2004
How to Cite
Zaari, N., Rajagopalan, P., Kim, S. K., Engler, A. J. and Wong, J. Y. (2004), Photopolymerization in Microfluidic Gradient Generators: Microscale Control of Substrate Compliance to Manipulate Cell Response. Adv. Mater., 16: 2133–2137. doi: 10.1002/adma.200400883
- Issue published online: 16 DEC 2004
- Article first published online: 16 DEC 2004
- Manuscript Accepted: 8 OCT 2004
- Manuscript Received: 4 JUN 2004
Integration of a microfluidic gradient generator and photopolymerization to fabricate novel hydrogels with tunable mechanical properties on the microscale (see Figure) is reported. This concept introduces a new class of materials that can be used to investigate the role of substrate elastic moduli in controlling cell morphology and cytoskeletal organization, and more generally, cell behavior.