The Patterning and Alignment of Muscle Cells Using the Selective Adhesion of Poly(oligoethylene glycol methyl ether methacrylate)-based ABA Block Copolymers

Authors


  • We thank Prof. E. W. Meijer (Laboratory of Organic and Macromolecular Chemistry, Eindhoven University of Technology) for helpful discussions and critical comments on the manuscript, Dr. P. E. L. G. Leclère (Service de Chimie des Matériaux Nouveaux, Université de Mons-Hainaut) for help with the AFM measurements, and Rob Petterson (Soft Tissue Biomechanics & Engineering, Eindhoven University of Technology) for the electrical stimulation experiments. We also thank the EPSRC (Grant no. GR/R37463) and NWO-CW for financial support.

Abstract

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The selective adhesion/delamination behavior of poly(oligoethylene glycol methyl ether methacrylate)-based ABA block copolymers is exploited to generate patterned substrates consisting of alternating lanes of glass and copolymer-coated gold. These patterns have been successfully used for the patterning and alignment of C2C12 muscle cells. The parallel-oriented myotubes (see Figure) contract upon electrical stimulation, forming a promising 2D in-vitro model for muscles.

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