Laser Scanning Lithography for Surface Micropatterning on Hydrogels


  • The authors acknowledge the NIH and the NSF for funding this work.


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A versatile confocal-based laser scanning lithographic method for controlled, high-fidelity two-dimensional and three-dimensional surface patterning of deformable, solvated, photoactive substrates is presented (see Figure; scale bars represent 200 μm). Patterned substrates functionalized with gradients of different cell-adhesive ligands can be fabricated, providing a tool for investigating cell–biomaterial interactions and the structure–function relationships of tissues.