Nanoscale ZnO-Enhanced Fluorescence Detection of Protein Interactions


  • We acknowledge Dr. C. Reddy for the use of the confocal and fluorescence microscope and thank E. Kunze and S. Magargee for helpful discussions regarding fluorescence microscopy measurements. J. H. acknowledges partial support of this work by the Huck Institutes of the Life Sciences and the Materials Research Institute at the Pennsylvania State University.


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Enhanced fluorescence emission is detected from interacting protein pairs of dichlorotriazinylaminofluorescein-streptavidin and biotinylated bovine serum albumin (see figure) that are adsorbed onto periodically spaced, square-patterned ZnO nanostructures. This florescence-enhancement capability of nanoscale ZnO and its potential easy integration into biosensor arrays may allow ultrasensitive protein detection, which is needed in the areas of biomedical research and in large-scale testing and screening.