We acknowledge Dr. C. Reddy for the use of the confocal and fluorescence microscope and thank E. Kunze and S. Magargee for helpful discussions regarding fluorescence microscopy measurements. J. H. acknowledges partial support of this work by the Huck Institutes of the Life Sciences and the Materials Research Institute at the Pennsylvania State University.
Nanoscale ZnO-Enhanced Fluorescence Detection of Protein Interactions†
Article first published online: 15 SEP 2006
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 18, Issue 20, pages 2685–2690, October, 2006
How to Cite
Dorfman, A., Kumar, N. and Hahm, J. (2006), Nanoscale ZnO-Enhanced Fluorescence Detection of Protein Interactions. Adv. Mater., 18: 2685–2690. doi: 10.1002/adma.200502616
- Issue published online: 10 OCT 2006
- Article first published online: 15 SEP 2006
- Manuscript Revised: 22 JUN 2006
- Manuscript Received: 6 DEC 2005
- Huck Institutes of the Life Sciences
- Materials Research Institute at the Pennsylvania State University
- Zinc oxide
Enhanced fluorescence emission is detected from interacting protein pairs of dichlorotriazinylaminofluorescein-streptavidin and biotinylated bovine serum albumin (see figure) that are adsorbed onto periodically spaced, square-patterned ZnO nanostructures. This florescence-enhancement capability of nanoscale ZnO and its potential easy integration into biosensor arrays may allow ultrasensitive protein detection, which is needed in the areas of biomedical research and in large-scale testing and screening.