C. A. M. acknowledges NSF, AFSOR, DARPA, and NIH for supporting this work. C. A. M. is grateful for a NIH Director's Pioneer Award. S. W. L and B.-K. O acknowledge Korea Research Foundation Grant funded by Korea Government for support of a postdoctoral fellowship (grant no. M01-2003-000-20278-0, M01-2003-000-20168-0). Supporting Information is available online from Wiley InterScience or from the author.
Biologically Active Protein Nanoarrays Generated Using Parallel Dip-Pen Nanolithography†
Article first published online: 28 MAR 2006
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 18, Issue 9, pages 1133–1136, May, 2006
How to Cite
Lee, S. W., Oh, B.-K., Sanedrin, R. G., Salaita, K., Fujigaya, T. and Mirkin, C. A. (2006), Biologically Active Protein Nanoarrays Generated Using Parallel Dip-Pen Nanolithography. Adv. Mater., 18: 1133–1136. doi: 10.1002/adma.200600070
- Issue published online: 24 APR 2006
- Article first published online: 28 MAR 2006
- Manuscript Accepted: 9 FEB 2006
- Manuscript Received: 11 JAN 2006
- Patterning, surface;
- Protein immobilization
Amine-active N-hydroxysuccinimide-terminated alkyl thiol templates are generated using parallel dip-pen nanolithography (DPN) and are used to covalently couple protein A/G. The protein arrays generated (see figure) are used to capture antibodies through affinity binding, while preserving their biological recognition properties. The versatility of the parallel DPN method for making many similar structures in a relatively high-throughput manner (14 000 dots in 10 min) is described.