Bone Formation Mediated by Synergy-Acting Growth Factors Embedded in a Polyelectrolyte Multilayer Film

Authors

  • A. Dierich,

    1. Institut de Génétique et de Biologie Moléculaire et, Cellulaire (IGBMC), Institut Clinique de la Souris (ICS), CNRS/INSERM/ULP, Collège de France, BP 10142, Strasbourg, France
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  • E. Le Guen,

    1. Faculté de Chirurgie Dentaire de L'Université Louis Pasteur, Institut National de la Santé et de la Recherche Médicale, Unité 595, Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cedex, France
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  • N. Messaddeq,

    1. Institut de Génétique et de Biologie Moléculaire et, Cellulaire (IGBMC), Institut Clinique de la Souris (ICS), CNRS/INSERM/ULP, Collège de France, BP 10142, Strasbourg, France
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  • J.-F. Stoltz,

    1. Unite Mixte de Recherches 7561, Centre National de la Recherche Scientifique, Université Henri Poincaré Nancy 1, Faculté de Médecine, Vandoeuvre les Nancy, 54000 Nancy, France
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  • P. Netter,

    1. Unite Mixte de Recherches 7561, Centre National de la Recherche Scientifique, Université Henri Poincaré Nancy 1, Faculté de Médecine, Vandoeuvre les Nancy, 54000 Nancy, France
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  • P. Schaaf,

    1. Institut Charles Sadron (CNRS/ULP), 6 rue Boussingault, 67083 Strasbourg Cedex, France
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  • J.-C. Voegel,

    1. Faculté de Chirurgie Dentaire de L'Université Louis Pasteur, Institut National de la Santé et de la Recherche Médicale, Unité 595, Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cedex, France
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  • N. Benkirane-Jessel

    1. Faculté de Chirurgie Dentaire de L'Université Louis Pasteur, Institut National de la Santé et de la Recherche Médicale, Unité 595, Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cedex, France
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  • We thank Professor Pierre Chambon (IGBMC, ICS, Collège de France, Strasbourg) for helpful discussions. This work was supported by the “Fondation de L'Avenir pour la Recherche Médicale Appliquée” and the “Ligue contre le Cancer (Région Alsace)”. N.J. is indebted to Bernard Senger for many fruitful and stimulating discussions and to Raphael Darcy (UCD, Dublin) for the generous gift of Cyclodextrin. Supporting Information is available online from Wiley InterScience or from the author.

Abstract

original image

A bifunctionalized polyelectrolyte multilayer film (PEM film) that can drive embryonic stem cells to cartilage or bone differentiation is presented. A model system is constructed from a PEM film into which transforming growth factor β1 and bone morphogenetic protein has been embedded. The synergy between both factors is required for the film to be bioactive. Osteogenesis in embryonic stem cells is induced by the film (see figure).

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